The NMR Bruker Ascend^TM 400 MHz Spectrometer is intended for rapid acquisition of routine 1D and 2D NMR experiments. This Spectrometer is perfectly equipped to tackle a wide variety of research problems and is mainly dedicated to chemistry studies. The Ascend^TM magnet incorporates the key technologies of the UltraShield^TM Plus magnets. The Avance^TM III platform has two channels (SGU1 and SGU2), 50 W ¹H/¹⁹F amplifier, 135 W X amplifier and MULTILINK^TM HPPR/2 preamplifiers. The Bruker Ascend^TM 400 MHz is equipped with a 5 mm BBFO^PLUS probe with ²H "lock" channel and z gradient. The spectrometer is also equipped with a control temperature unit prepared to work at temperatures ranging from -50 °C to +50 °C.

Specifications

• UltraShield^TM Plus 9.4T magnet
• 5 mm BBFO^PLUS probe, optimized for X-nuclei direct observation. Broad band in a frequency range between ³¹P and ¹⁵N. This probe has ²H "lock" channel and z gradient that allows us to carry out 2D spectroscopy and hetero correlation ¹⁹F/¹H and ¹H/¹⁹F. The BBFO^PLUS probe also includes an automated tuning and matching device (ATM).
• Control temperature unit (from -50 °C to 50 °C).

Experiments

• 1D spectra 1H, ¹³C, ³¹P, ²⁹Si…
• 2D correlation ¹H-¹H: COSY, NOESY, ROESY, TOCSY
• 2D correlation ¹H-X: HSQC, HMBC, HMQC, H2BC
• Diffusion 1D, 2D DOSY

Applications

• Structure verification and elucidation
• Molecular mobility: determination of conformation in solution
• Determination of enantiomeric excess
• Kinetic and temperature studies of reaction mixtures

Detection and Structural Identification of Dissolved Organic Matter in Antarctic Glacial Ice at Natural Abundance by SPR-W5-WATERGATE 1H NMR Spectroscopy
Brent G. Environ. Sci. Technol., 2011, 45 (11), pp 4710–4717

Kinetic Understanding Using NMR Reaction Profiling
S. Flavien et al. Org. Process Res. Dev. 2012, 16, 61–64

Chiral reagents for the determination of enantiomeric excess and absolute configuration using NMR spectroscopy
TJ Wenzel, JD Wilcox – Chirality, 2003