The NMR Bruker AVANCE^TM 600 MHz Spectrometer is mainly intended for biomedical research purposes. The magnet incorporates the key technologies of the UltraShield^TM Plus magnets. The AVANCE^TM III platform has three channels (SGU1, SGU2 and SGU3), the SGU1 to observe and decouple 1H and SGU2 and SGU3 for a frequency range between ¹⁰⁹Ag and ³¹P. The magnet is equipped with 4 different probes, the 5 mm BBFO probe, 5 mm TXI 1H/13C/31P probe, HR-MAS 1H-13C-31P probe and a TCI CryoProbe Prodigy. The CryoProbe Prodigy is geared for handling either very low concentration samples or those with very high molecular weights and allows you to work at 0°C–80°C sample temperature range. The spectrometer is also equipped with a control temperature unit prepared to work at VT range (-50 °C to +50 °C).

Specifications

• UltraShield^TM Plus 9.4T magnet
• 5 mm BBFO^PLUS probe, optimized for X-nuclei direct observation in a frequency range between ³¹P and ^15N. This probe has ²H "lock" and a z gradient, as well as automated tuning and matching (ATM).
• 5 mm TXI probe, optimized for higher sensitivity ¹H-nuclei observation. While the inner coil is optimized for 1H observation, the outer coil is optimized for decoupling of ¹³C and ³¹P. It has ²H "lock" channel and z gradient, and automated tuning and matching device (ATM).
• 4 mm TXI HR-MAS probe, optimized for ¹H-¹³C-³¹P observation. This probe has ²H "lock" channel and is configured with a Z-gradient aligned along the magic angle.
• TCI CryoProbe Prodigy, uses nitrogen-cooled RF coils and preamplifiers for ¹H, ²H and ¹³C, delivering a 2.5x ¹H sensitivity boost. The probe is ATM compatible and has ²H "lock" channel and Z-gradient.
• Control temperature unit (from -50 °C to 50 °C).

Experiments

• 1D spectroscopy: 1H, ¹³C, ³¹P, ²⁹Si…
• 2D correlation ¹H-¹H: COSY, NOESY, ROESY, TOCSY
• 2D correlation ¹H-X: HSQC, HMBC, HMQC, H2BC
• Diffusion 1D, 2D DOSY

Applications In Biomedicine

Metabonomics and Metabolomics

The Handbook of Metabonomics and Metabolomics.
J.C Lindon et al. 2007, 14, 373–410

High-Resolution 1H-RMN Spectroscopy of Blood Plasma for Metabolic Studies
A. Ron et al, Clin. Chem. 1994, 40/7, 1245-1250

Elevated CSF N-acetylaspartylglutamate in patients with free sialic acid storage diseases
F. Mochel et al, Neurology. 2012, 74, 302-305

1H HR-MAS and genomic analysis of human tumor biopsies discriminate between high and low grade astrocytomas.
V. Righi et al, NMR in Biomedicine. 2009, 22, 629-637

HR MAS MR spectroscopy in metabolic characterization of cancer
S. Moestue et al. Curr Top Med Chem. 2011;11(1):2-26. Review

In vivo Metabolism using enriched stable isotope tracers.

13C NMR and cerebral biochemistry
S. Cerdan et al, NMR Biomed. 2003, 16, 301-302

13C NMR tracers in neurochemistry: implications for molecular imaging.
T.B. Rodrigues et al. Q J Nucl Med Mol Imaging. 2009 Dec;53(6):631-45. Review.

Applications In Chemistry

NMR spectroscopy techniques for screening and identifying ligand binding to protein receptors
B. Meyer et al. Angew Chem Int Ed Engl. 2003 Feb 24;42(8):864-90.

Accurate nucleic acid concentrations by nuclear magnetic resonance
M. J. Cavaluzzi et al. Anal Biochem. 2002 Sep 15;308(2):373-80.

DNA-nanotube-induced alignment of membrane proteins for NMR structure determination
S. M. Douglas et al. Proc Natl Acad Sci U S A. 2007 Apr 17;104(16):6644-8. Epub 2007 Apr 2.