Nikon Eclipse Ti TIRF Microscope

Total Internal Reflection Fluorescence (TIRF) microscopy combines confocal-like contrast with the sensitivity of widefield microscopy for visualising of near-membrane structures. TIRF microscopy angles the illumination so that instead of passing through the sample, it reflects internally between the objective and the coverslip and then back into the objective. Only the part of the sample that is in close contact with the coverslip is illumination by an effervescent wave generated from the coverslip and extending up to ~150nm into the sample. The end result is the ability to visualise membrane bound proteins, receptors, vesicles and the cortical cytoskeleton without the problems of out of focus-light as the rest of the cell is not illuminated. The combination of better sensitivity and limited illumination make this a much better choice for near-membrane imaging that confocal microscopy, especially when dealing with weakly fluorescent or easily bleached samples. The Nikon Eclipse Ti TIRF microscope combines an extremely sensitive Andor EM-CCD camera with laser-based illumination and a motorised TIRF mirror to enable true multi-colour TIRF imaging.

Specifications 

  • Inverted Nikon Ti Eclipse fully motorised microscope.
  • Motorised TIRF mirror
  • Multipass TIRF dichroic filter (allows high-speed multicolour TIRF)
  • Andor iXon3 897 EM-CCD camera
  • Microscope enclosure with temperature, humidity and CO2 control.
  • Objective lenses:
    • Plan Apo Oil immersion: 100x (1.49 NA) TIRF
    • Plan Fluor 40x (0.75 NA)
    • Plan Apo 10x (0.45 NA).
  • 4 Lasers in the visible range:
    • Violet 405nm
    • Argon-Ion 457nm, 477nm, 488nm, 514nm
    • Yellow Diode 561nm
    • Red 647nm
  • High precision motorized stage.
  • Nikon PFS (Perfect Focus System) for improved stability

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